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Alignments of data from these re- sequenced organisms is a relatively simple method of detecting variation in samples. There are certain instances (such as new genes in the sequenced sample that are not found in the existing reference sequence) that can not be detected by alignment alone; however, while other approaches, such as de novo assembly, are potentially more powerful, they are also much harder or, for some organisms, impossible to achieve with current sequencing methods. Next- generation sequencing generally produces short reads or short read pairs, meaning short sequences of < ~2. Sanger sequencing, which cover ~1. To compare the DNA of the sequenced sample to its reference sequence, we need to find the corresponding part of that sequence for each read in our sequencing data. This is called aligning or mapping the reads against the reference sequence. Once this is done, we can look for variation (e. SNPs) within the sample. This poses a number of problems: The short reads do not come with position information, that is, we do not know what part of the genome they came from; we need to use the sequence of the read itself to find the corresponding region in the reference sequence. The reference sequence can be quite long (~3 billion bases for human), making it a daunting task to find a matching region. Since our reads are short, there may be several, equally likely places in the reference sequence from which they could have been read. This is especially true for repetitive regions. If we were only looking for perfect matches to the reference, we would never see any variation. Therefore, we need to allow some mismatches and small structural variation (In. Dels) in our reads. Any sequencing technology produces errors. Similar to the "real" variation, we need to tolerate a low level of sequencing errors in our reads, and separate them from the "real" variation later. We need to do that for each of the millions of reads in our sequencing data. Galaxy is an open, web-based platform for accessible, reproducible. Galaxy Project @ Johns Hopkins is hiring – Software engineers and post-docs. A comprehensive review of RNA-seq methodologies. Next-generation sequencing is rapidly becoming the method of. Bowtie is best suited to aligning genomic DNA. Discover the broad range of experiments you can perform with next-generation sequencing. Select the best. Next-gen sequencing generates masses of DNA. Trends in Genetics All Journals. Ten years ago next-generation sequencing. Base-calling software software to analyze the raw data produced by automated. The DNA Sequencing Facility offers all-inclusive next generation sequencing. Terminals and software are available in our computer lab for clients of UWBC. Short reads[edit]Raw short reads often come in (or can be converted into) a file format called FASTQ.[2] It is a plain text format, containing the sequence and quality scores for every read, where each single read normally occupies four consecutive lines. CTGATGTGCCGCCTCACTTCGGTGGT. DDDDDH8< BAHG@BHGIHIII> (. TGATGTGCCGCCTCACTACGGTGGTG. FHHHHHJIJIJIJIIIJJIIJGIGII. The four lines are: The name/ID of the read, preceded by a "@". For read pairs, there will be two entries with that name, either in the same or a second FASTQ file. The sequence of the read. A "+" sign. In very old FASTQ files, this is followed by the read name from the first line. Today, this line is present for historical reasons backwards compatibility only. The quality scores of the bases from line 2. The scores are generated by the sequencing machine, and encoded as ASCII (3. The line should have the same length as line 2, as there is one quality score per base. Alignment[edit]For each of the short reads in the FASTQ file, a corresponding location in the reference sequence (or that no such region exists) needs to be determined. Best Next Generation Sequencing Software Wikipedia ProcesoThis is achieved by comparing the sequence of the read to that of the reference sequence. A mapping algorithm will try to locate a (hopefully unique) location in the reference sequence that matches the read, while tolerating a certain amount of mismatch to allow subsequence variation detection. Reads aligned (mapped) to a reference sequence will look like this: GCTGATGTGCCGCCTCACTTCGGTGGTGAGGTG Reference sequence. The Impact of Next-Generation Sequencing on Genomics. and the potential impact of next-generation sequencing. provides the best data qual ity as a result of. CTGATGTGCCGCCTCACTTCGGTGGT Short read 1. TGATGTGCCGCCTCACTACGGTGGTG Short read 2. GATGTGCCGCCTCACTTCGGTGGTGA Short read 3. GCTGATGTGCCGCCTCACTACGGTG Short read 4. GCTGATGTGCCGCCTCACTACGGTG Short read 5. You can see the reference sequence on the top row, and five short reads stacked below; this is called a pileup. While two of the reads are a perfect match to the reference, the three other reads show a mismatch each, highlighted in red ("A" in the read, instead of "T" in the reference). Since there are multiple reads showing the mismatch, at the same position, with the same difference, one could conclude that it is an actual genetic difference (point mutation or SNP), rather than a sequencing error or mismapping. Mapping algorithms[edit]There are several alignment algorithms in existence; you can find an (incomplete) list further down in software packages. Some notes on mapping algorithms: The reference sequence, the short reads, or both, are often pre- processed into an indexed form for rapid searching. See BWT)Sources of errors[edit]There are several potential sources for errors in an alignment, including (but not limited to): PCR artifacts. Many NGS methods involve one or multiple PCR steps. PCR errors will show as mismatches in the alignment, and especially errors in early PCR rounds will show up in multiple reads, falsely suggesting genetic variation in the sample. A related error would be PCR duplicates, where the same read pair occurs multiple times, skewing coverage calculations in the alignment. Sequencing errors. The sequencing machine can make an erroneous call, either for physical reasons (e. Illumina slide), or due to properties of the sequenced DNA (e. As sequencing errors are often random, they can be filtered out as singleton reads during variant calling. Mapping errors. The mapping algorithm can map a read to the wrong location in the reference. This often happens around repeats or other low- complexity regions. Alignment types[edit]Alignments can be used for different purposes: Whole- genome sequencing. This would be the "default" use; sequence all DNA from an organism and map it to the appropriate reference sequence, to find genetic variation. Exome sequencing. For large genomes (e. DNA before sequencing. This will return sequencing data for most of the genes, at a fraction of the cost. Transcriptome sequencing (RNA- Seq). Sequencing of the transcriptome, that is, of the RNA present in the sample. This can show which genes are transcribed in the sample, and help fine- tune gene annotation (exon boundaries etc.). Mapping can be done either to the full reference sequence, or to a special "transcriptome reference". Ch. IP- Seq (Protein- DNA interaction). The SAM/BAM format[edit]The SAM/BAM format has emerged as the de facto standard format for short read alignments. SAM[3] is the plain- text version of the binary, compressed BAM format. They can be converted into one another by the name- giving samtools[4] command- line tool. BAM (without alignment position data) is increasingly used as a space- saving alternative to FASTQ files for containing the short raw read data, and all current alignment software can generate SAM/BAM as an output format. Once in BAM format, the file can be indexed, giving quick access to any region of the reference sequence. Subsequently, using samtools or other software, BAM files can be analysed (e. PCR duplicates, local realignment, base quality recomputation), or used to call variation, either small (SNPs, short In. Dels) or large (inversions, tandem duplications, deletions, translocations). BAM files can be visualised using tools like Artemis, ACT, or Look. Seq[5]. Last but not least, alignments in BAM format can be used to "morph" the reference sequence to correspond to the short read data with ICORN[6]; this can be useful to get an actual DNA sequence for a sample, or to construct a new reference sequence based on a closely related species. Other useful SAM/BAM- related software includes: SAM/BAM tools examples[edit]Convert SAM to BAM format. S aln. sam > aln. Lists of Genomics Software/Service Providers. Genome. Source contains the information found here, but in a searchable format. I also hope to keep a running tally of any insertions and deletions (Indels) that I make to both sites. As I get used to posting on the new format, I hope to use it to share any new developments in genomics and personalized medicine that I learn about as I work my day job at Bio. Chain. Please also email me at geoffhrouth@gmail. Torrent Search Engine | 1. Due to our old index being blocked by search engines new users were having issues finding website. We developed new index page that contains only search so we don't get blocked by search engines in the future. Old home page is here, you can also click "full home page" link in the header or 1. List of conspiracy theories - Wikipedia. For a list of genuine conspiracies, see List of political conspiracies. There are many unproven conspiracy theories with varying degrees of popularity, frequently related to clandestine government plans and elaborate murder plots. Conspiracy theories usually deny consensus or cannot be proven using the historical or scientific method, and are not to be confused with research concerning verified conspiracies such as Germany's pretense for invading Poland in World War II. Conspiracy theory is often considered the opposite of institutional analysis. Ethnicity, race, and religion. Antisemitism. Since at least the Middle Ages, antisemitism has featured elements of conspiracy theory. Blast Loading and Blast Effects on Structures – An Overview T. Ngo, P. Mendis, A. Gupta & J. 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In medieval Europe it was widely believed that Jews poisoned wells, had been responsible for the death of Jesus, and ritually consumed the blood of Christians. The second half of the 1. Jews and/or Freemasons were plotting to establish control over the world. Forged evidence has been presented to spread the notion the Jews were responsible for the propagation of Communism, the most notorious example being The Protocols of the Elders of Zion (1. Such antisemitic conspiracy theories became central to the worldview of Adolf Hitler. Antisemitic theories persist today in notions concerning banking,[2] Hollywood, the news media and a purported Zionist Occupation Government.[3][4][5]Holocaust denial. Holocaust denial is also considered an antisemitic conspiracy theory because of its position that the Holocaust is a hoax designed to advance the interests of Jews and justify the creation of the State of Israel.[6][7] Notable Holocaust deniers include former Iranian President Mahmoud Ahmedinejad,[8] the convicted Germar Rudolf[9] and the discredited author David Irving.[1. Anti- Armenianism. Conspiracy theories which allege that the Armenians wield secret political power are prevalent in Azerbaijan,[1. President Ilham Aliyev.[1. Turkish Foreign Minister Mevlüt Çavuşoğlu has claimed that the Russian media is run by Armenians.[1. American writer and disbarred lawyer Samuel Weems[1. Armenian Genocide was a hoax designed to defraud Christian nations of billions of dollars, and that the Armenian Church instigates terrorist attacks.[1. Filmmaker Davud Imanov has accused the Armenians of plotting against Azerbaijan and has claimed that the Karabakh movement was a plot by the CIA to destroy the Soviet Union.[1. Journalist Arzu Geybulla has drawn attention to the way in which such theories are used to stifle political dissent.[1. Anti- Baha'ism. Iran's Baha'i minority has been the target of conspiracy theories alleging involvement with hostile powers. Iranian government officials and others have claimed that Bahá'ís have been agents variously of Russian imperialism, British colonialism, American expansionism and Zionism.[2. An apocryphal and historically- inaccurate book published in Iran, entitled The Memoirs of Count Dolgoruki, details a theory that the Bahá'ís intend to destroy Islam. Such anti- Bahá'í accusations have been dismissed as having no factual foundation.[2. Anti- Catholicism. Anti- Catholicparanoia has featured in the Protestant mind since the Reformation. Conspiracy theories have taken many forms, including the 1. Popish Plot allegations,[2. William Blackstone that Catholics posed a secret threat to Britain, and numerous writings by authors such as Rebecca Reed, Avro Manhattan, Jack Chick and Alberto Rivera. Theorists often claim that the pope is the Antichrist, or they accuse Catholics of suppressing evidence incompatible with Church teachings and engaging in secret evil rituals, crimes and other plots. Fears of a Catholic takeover of the US have been especially persistent,[2. Catholic immigration in the 1. Ku Klux Klanpropaganda.[2. Such fears have attached to Catholic political candidates such as Al Smith[3. John F. Kennedy.[3. The papacy. Pope John Paul I died in September 1. The timing of his death and the Vatican's alleged difficulties with ceremonial and legal death procedures has fostered several conspiracy theories. The elderly Pope Benedict XVI's resignation in February 2. Italian publications such as La Repubblica and Panorama that he resigned in order to avoid an alleged scandal involving an underground gay Catholic network.[3. Antichrist. Apocalyptic prophecies, particularly Christian claims about the End Times, have inspired a range of conspiracy theories. Many of these cite the Antichrist, a leader who will supposedly create an oppressive world empire. Countless figures have been called Antichrist, including Holy Roman Emperor Frederick II, Russian emperor Peter the Great, Saladin, pope John XXII, and Benito Mussolini.[3. The Bible. Bible conspiracy theories posit that much of what is known about the Bible is false. These theories often claim that Jesus had a wife and children and that he did not die on the cross. Various groups both real (such as the Vatican) and fake (such as the Priory of Sion) are said to suppress relevant information concerning, for example, the dating of the Turin Shroud. Theorists also allege that books which originally belonged in the Bible have been censored.[3. Islam. War against Islam"War against Islam" is a conspiracy theory in Islamist discourse which describes an alleged plot to either harm or annihilate the social system within Islam. The perpetrators of this conspiracy are alleged to be non- Muslims and "false Muslims", allegedly in collusion with political actors in the Western world. The "War against Islam" theory is often used in order to refer to modern social problems and changes, but the Crusades are often seen as its starting point.[3. Love Jihad. Love Jihad, also called Romeo Jihad, refers to a conspiracy theory concerning Muslim males who are said to target non- Muslim girls for conversion to Islam by feigning love.[4. Racism. Black genocide. In the United States, black genocide conspiracy theory[4. African Americans are the victims of genocide instituted by white Americans. Lynchings and racial discrimination were formally described as genocide by the Civil Rights Congress in 1. Malcolm X also talked about "black genocide" in the early 1. Public funding of the Pill was also described as "black genocide" at the first Black Power Conference, in 1. In 1. 97. 0, after abortion was more widely legalized, some black militants depicted abortion as being part of the conspiracy.[4. Lies of Babylon. Some Rastafari maintain the view that a whiteracistpatriarchy ("Babylon") controls the world in order to oppress black people.[5. They believe that Emperor Haile Selassie of Ethiopia did not die in 1. Rastafari movement.[5. The Plan. In some U. S. cities that are governed by African American majorities, such as Washington, D. C., a persistent conspiracy theory holds that white Americans are plotting to take over those cities. White genocide. White genocide conspiracy theory is a white nationalist notion that immigration, integration, low fertility rates and abortion are being promoted in predominantly white countries in order to turn white people into a minority or cause their extinction.[5. Deaths and disappearances. Conspiracy theories frequently emerge following the deaths of prominent people. The best known of these are the theories concerning the assassination of John F. Kennedy in 1. 96. Central to many of these theories is a claim a lone gunman could not have caused Kennedy's injuries. Other theories allege that President Johnson was involved in the killing. The deaths of establishment figures of all types attract conspiracy theorists, including, for example, the deaths of Martin Luther King, Jr., Eric V, Dmitry Ivanovich, Sheikh Rahman, Yitzhak Rabin,[5. Zachary Taylor,[5. George S. Patton,[6. Princess Diana,[6. Dag Hammarskjöld,[6. 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Per controllare quale versione del pacchetto software Hercules avete installato dovete aprire il pannello di controllo del driver (doppio click sull'icona a forma di disco presente nella notification area in basso a destra vicino l'orologio di Windows) e leggere dove indicato in rosso sotto: Se non capite perchè quando attivate il microfono il volume dell'uscita della console si abbassa, basta deselezionare l'opzione indicata sotto: LATENZASe vi dovesse "saltare" o "gracchiare" l'audio in uscita (dropout) andate nel pannello del driver Hercules, tab AVANZATE o ASIO o AUDIO e provate ad AUMENTARE il valore dell'ASIO Buffer size fino a che il problema non sparisce. PC viewtopic. php? The manuals list of Synti Groep Below you find a full summary of all the owners manuals, service manuals, schematics and other documentation we have available of. Top VIdeos. Warning: Invalid argument supplied for foreach() in /srv/users/serverpilot/apps/jujaitaly/public/index.php on line 447. · 웹 해킹 - 웹 페이지 관련 구성 파일 이름목록 웹 해킹 / Security_Study. · Driver aggiornati periferiche Hercules Pacchetto software versione 2016_HDJS_3 per PC Windows 10 / 8.1 / 8 / 7 / Vista (32/64-bit) Download -> http://ts.hercules.com.PC nuovo e più potente. AUTO GAIN e SYNCOgni volta che metto una canzone nuova i gain tornano al centro e mi tocca girare la manopola per tornare alla regolazione che io ho deciso. I gain virtuali sul software si spostano sempre ogni volta che si cambia canzone grazie alla funzione AUTO GAIN che adatta il valore del guadagno (gain) per ogni canzone, in modo che si possa suonare una canzone dopo l'altra senza dover appunto variare la manopola del gain ad ogni cambio traccia. Quando si vuole variare il GAIN dal controller la manopola agisce solo quando il valore della manopola "aggangia" quello del software, in modo da evitare sbalzi di volume. Funziona allo stesso modo per i fader del pitch (tempo), basta spingere il tasto SYNC e provare poi a muovere il pitch fisico per accorgersene. Shop HP’s Online Store for all your computer hardware, accessories, printer needs & the best deals. Free Shipping + Easy Returns. Shop now. In this guide I explain how to disassemble a Compaq Presario F700 notebook. The guide might work for some other Compaq and HP models. I think the disassembly steps. Update : 2 : If you are looking for a guide to Dual Boot Windows Vista and Mac OSX Leopard, please follow this guide Hack Attack : Dual Boot Leopard and Windows Vista. How to disassemble Compaq Presario F7. In this guide I explain how to disassemble a Compaq Presario F7. The guide might work for some other Compaq and HP models. I think the disassembly steps will be very similar for Compaq Presario F5. HP G6. 00. 0 notebook PC. If this guide works for your notebook, please mention the model name in the comments below. In this particular guide I’m taking apart a Presario F7. US notebook. Before you start, turn off the laptop, unplug the power adapter and remove the battery. COMPAQ PRESARIO F7. DISASSEMBLY GUIDE. STEP 1. Remove three screws securing the memory cover and two screws securing the hard drive cover. Remove both covers. STEP 2. Lift up the right side of the hard drive by the plastic tab to disconnect it from the motherboard. Remove the hard drive. STEP 3. Remove both memory modules. UPDATE (January, 2. Compaq Presario F7. GB RAM max (1. GB per slot). I just tested a Compaq Presario F7. Windows 7 Home Premium 3. OS) with two 2. GB modules. I used Crucial DDR2 PC- 6. After I installed two 2. GB modules the laptop started properly. In system properties I can see 3. GB total RAM reported. The BIOS shows all 4. GB. I think that 3. GB is only limitation of a 3. OS. Most likely if you install a 6. OS, you can use all 4. GB. Were you able to upgrade your Presario F7. GB? Please leave a comment. STEP 4. Disconnect both antenna cables from the wireless card. Remove two screws securing the wireless card in the top left and right corners. Pull the wireless card from the slot and remove it. STEP 5. Remove one screw securing the CD/DVD drive and pull the drive from the notebook. Remove the drive. STEP 6. Remove all marked screws from the bottom of the notebook. Yellow screws securing the switch cover (keyboard cover). Green screws securing the keyboard. STEP 7. Start lifting up the switch cover as it shown on the picture below. You’ll have to work with your fingers to disengage small plastic latches holding the switch cover. Be careful, there is a thin ribbon cable running from the switch cover to the motherboard. STEP 8. Now you can lift up the top side of the keyboard and access the connector underneath the keyboard. The keyboard cable connector is located above the touch pad. Before you pull the cable and remove the keyboard you have to unlock the connector. Move both sides of the locking tab about 1- 2 millimeter up and release the keyboard cable. Now you can pull the cable and remove the keyboard. STEP 9. Disconnect the switch cover cable from the motherboard. Remove the cover. STEP 1. 0. Unplug the LCD cable from the motherboard. Release the wireless card antenna cables. Pull them through a hole in the motherboard. Remove four screws securing the display panel. STEP 1. 1. Lift up and remove the display panel assembly. In the next guide I will explain how to access and remove the LCD screen with inverter board. STEP 1. 2. Remove four screws securing the top cover assembly. Disconnect the power button board cable. Disconnect the touch pad cable. STEP 1. 3. Remove all screws securing the top cover assembly from the bottom of the notebook. Do not forget three (green) screws in the CD/DVD drive bay. Remove two hex studs located close to the memory slots. By the way, the RTC (CMOS) battery can be accessed when you remove the memory cover. STEP 1. 4. Start separating the top cover assembly from the bottom assembly. You’ll have to work with your fingers to disengage plastic latches securing the top cover. Carefully lift up and remove the top cover assembly. STEP 1. 5. Now I’m going to remove the motherboard. Remove one screw securing the motherboard. In other Compaq/HP models there could be more then one screw securing the motherboard. STEP 1. 6. Lift up the right side of the motherboard as it shown on the picture below. Before you remove the motherboard from the base assembly you have to disconnect the audio board cable. STEP 1. 7. Remove the motherboard and turn it upside down. There are two more cables to disconnect. These cables connect the motherboard to the USB/Power connector board. Finally, the motherboard is removed. As you see on the picture below, the audio board and USB/power connector board are secured to the base assembly. The motherboard has been removed. Now you can access and replace the cooling fan if you have to. You can access and replace the processor if you remove the heat sink. You can support my work! It takes hours to create one disassembly guide and only a minute to donate. |
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November 2017
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